<div dir="ltr"><p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif" style="">Dear MARMAM members,</font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif"> </font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN" style=""><font face="arial, sans-serif">On
behalf of all the authors, I would like to share with you our last paper published
in Marine Genomics:</font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN" style=""><font face="arial, sans-serif"> </font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN" style=""><font face="arial, sans-serif">“Skin
distress associated with xenobiotics exposure: An epigenetic study in the
Mediterranean fin whale (Balaenoptera physalus)”</font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><font face="arial, sans-serif">Authors: Annalaura
Mancia, Luigi Abelli, Maria Cristina Fossi, Cristina Panti</font></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><a class="gmail-doi" href="https://doi.org/10.1016/j.margen.2020.100822" target="_blank" rel="noreferrer noopener" title="Persistent link using digital object identifier" style="box-sizing:border-box;margin:0px;padding:0px;background-color:transparent;text-decoration-line:none;color:rgb(12,125,187);word-break:break-word;font-family:NexusSans,Arial,Helvetica,"Lucida Sans Unicode","Microsoft Sans Serif","Segoe UI Symbol",STIXGeneral,"Cambria Math","Arial Unicode MS",sans-serif;font-size:14px">https://doi.org/10.1016/j.margen.2020.100822</a></p>

<br><p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial">The publication can be found at: <font face="arial, sans-serif"><a href="https://www.sciencedirect.com/science/article/pii/S1874778720300830">https://www.sciencedirect.com/science/article/pii/S1874778720300830</a></font></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><font face="arial, sans-serif"> </font></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif">Abstract:</font></span></p>

<p class="MsoNormal" style="margin:0cm;text-align:justify;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif">The phenotypic plasticity of
many organisms is mediated in part by epigenetics, the heritable changes in
gene activity that occur without any alterations to DNA sequence. A major
mechanism in epigenetics is the DNA methylation (DNAm). Hypo- and
hyper-methylation are generalized responses to control gene expression however
recent studies have demonstrated that classes of contaminants could mark
specific DNAm signatures, that could usefully signal prior environmental
exposure.</font></span></p>

<p class="MsoNormal" style="margin:0cm;text-align:justify;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif">We collected skin and blubber
from 6 free-ranging fin whale (Balaenoptera physalus) individuals sampled as a
part of a previous published study in the northern Mediterranean Sea. Genomic
DNA extracted from the skin of the fin whales and levels of contaminants
measured in the blubber of the same individuals were used for DNAm profiling
through reduced representation bisulfite sequencing (RRBS).</font></span></p>

<p class="MsoNormal" style="margin:0cm;text-align:justify;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif">We tested the hypothesis that
differences in the methylation patterns could be related to environmental
exposure to contaminants and load in the whale tissues.</font></span></p>

<p class="MsoNormal" style="margin:0cm;text-align:justify;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif">The aims of this study were to
determine the DNAm profiles of the methylation contexts (CpGs and non-CpGs) of
differently contaminated groups using the RRBS, and to identify potential
contaminant exposure related genes. Amount and proportion of methylcytosines in
CpG and non-CpG regions (CHH and CHG) was very similar across the 6 samples.
The proportion of methylcytosines sites in CpG was n = 32,682, the highest
among all the sequence contexts (n = 3216 in CHH; n = 1743 in CHG). The
majority of the methylcytosine occurred in the intron regions, followed by exon
and promoter regions in CpG, CHH and CHG. Gene Ontology results indicated that
DNAm affected genes that take place in cell differentiation and function in
cutaneous, vascular and nervous systems. The identification of cellular
response pathways allows a better understanding of the organism biological
reaction to a specific environmental challenge and the development of sensitive
tools based on the predictive responses. Eco-epigenetics analyses have an
extraordinary potential to address growing issues on pollution biomonitoring,
ecotoxicity assessment, conservation and management planning.</font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif"> </font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif"> </font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><font face="arial, sans-serif"><span lang="EN" style="">Thank
you for your interest in the paper!</span><span lang="EN-US" style=""></span></font></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><font face="arial, sans-serif"><span lang="EN" style=""> </span><span lang="EN-US" style=""></span></font></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif">Best regards,</font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif"> </font></span></p>

<p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif" style="">Cristina</font></span></p><p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif" style=""><br></font></span></p><p class="MsoNormal" style="margin:0cm;line-height:normal;background-image:initial;background-position:initial;background-size:initial;background-repeat:initial;background-origin:initial;background-clip:initial"><span lang="EN-US" style=""><font face="arial, sans-serif" style=""><br></font></span></p><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><p class="MsoNormal"><a name="SignatureSanitizer__MailAutoSig"><span style="font-size:10pt">><> ><>
><> ><> ><> ><> ><> ><>
><> ><> ><> ><><span></span></span></a></p>

<p class="MsoNormal"><span style="font-size:10pt">Cristina Panti, PhD<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt">Department of
Environmental, Earth and Physical Sciences<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt">University of Siena<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt">Via P.A. Mattioli, 4<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt">53100, Siena<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt">Italy<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt"> </span></p>

<p class="MsoNormal"><span style="font-size:10pt">Ph. +39 0577 232245<span></span></span></p>

<p class="MsoNormal"><span style="font-size:10pt">Fax. +39 0577 232930<span></span></span></p><p class="MsoNormal"><span style="font-size:10pt"><br></span></p><p class="MsoNormal"><a href="https://plasticbustersmpas.interreg-med.eu/" target="_blank">https://plasticbustersmpas.interreg-med.eu/</a><br></p><p class="MsoNormal"><a href="https://www.unisi.it/" target="_blank">https://www.unisi.it/</a><br></p><p class="MsoNormal"><br></p></div></div></div></div></div></div>