[MARMAM] New publication on Transcritpomic analysis of North Atlantic Right Whale

Annalaura MANCIA mncnlr at unife.it
Mon Dec 7 08:05:29 PST 2020


Dear MARMAM members,



On behalf of all the authors, I would like to share with you our last paper
published in Comparative Biochemistry and Physiology Part C: Toxicology &
Pharmacology:

*Transcriptome analysis of cadmium exposure in kidney fibroblast cells of
the North Atlantic Right Whale (Eubalaena glacialis) *by Ierardi JL, Velosa
A, Mancia A.

*Abstract:* An 8X15k oligonucleotide microarray was developed consisting of
2334 Eubalaena glacialis probes and 2166 Tursiops truncatus probes and used
to measure the effects, at transcriptomic level, of cadmium exposure in
right whale kidney fibroblast cells. Cells were exposed to three
concentrations (1 μM, 0.1 μM, and 0.01 μM) of cadmium chloride (CdCl2) for
three exposure times (1, 4, and 24 h). Cells exposed to 1 μM CdCl2 for 4 h
and 24 h showed upregulated genes involved in protection from metal
toxicity and oxidative stress, protein renaturation, apoptosis inhibition,
as well as several regulators of cellular processes. Downregulated genes
represented a suite of functions including cell proliferation,
transcription regulation, actin polymerization, and stress fiber synthesis.
The collection of differentially expressed genes in this study support
proposed mechanisms of cadmium-induced apoptosis such as ubiquitin
proteasome system disruption, Ca2+ homeostasis interference, mitochondrial
membrane potential collapse, reactive oxygen species (ROS) production, and
cell cycle arrest. The results also have confirmed the right whale
microarray as a reproducible tool in measuring differentiated gene
expression that could be a valuable asset for transcriptome analysis of
other baleen whales and potential health assessment protocols.

The full paper is available under the following link:
https://authors.elsevier.com/a/1cB-v5SMCg%7EbA1
<https://authors.elsevier.com/a/1cB-v5SMCg~bA1>

Best regards,
Annalaura Mancia

-- 
*Annalaura Mancia, PhD*
*Assistant Professor*
*University of Ferrara*
*Department of Life Sciences and Biotechnology*
*Via L. Borsari, 46*
*44121, Ferrara, FE, Italy*

*work: +390532455704*
*cell: +393421470120*
*fax: +390532455715*
*Email: annalaura.mancia at unife.it <annalaura.mancia at unife.it>*
*Skype: annalaura5*
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