[MARMAM] New publications: Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues

Wen-Ta Li heerolee1104 at gmail.com
Sat Oct 6 10:06:37 PDT 2018

Dear Colleague,

My co-authors and I are pleased to announce the new publication of the
following article in Journal of Visualized Experiments.

*Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean

Silver nanoparticles (AgNPs) have been extensively used in commercial
products, including textiles, cosmetics, and health care items, due
to their strong antimicrobial effects. They also may be released into the
environment and accumulate in the ocean. Therefore, AgNPs are the
major source of Ag contamination, and public awareness of the environmental
toxicity of Ag is increasing. Previous studies have demonstrated
the bioaccumulation (in producers) and magnification (in
consumers/predators) of Ag. Cetaceans, as the apex predators of ocean, may
been negatively affected by the Ag/Ag compounds. Although the
concentrations of Ag/Ag compounds in cetacean tissues can be measured by
inductively coupled plasma mass spectroscopy (ICP-MS), the use of ICP-MS is
limited by its high capital cost and the requirement for tissue
storage/preparation. Therefore, an autometallography (AMG) method with an
image quantitative analysis by using formalin-fixed, paraffin-
embedded (FFPE) tissue may be an adjuvant method to localize Ag
distribution at the suborgan level and estimate the Ag concentration in
cetacean tissues. The AMG positive signals are mainly brown to black
granules of various sizes in the cytoplasm of proximal renal tubular
epithelium, hepatocytes, and Kupffer cells. Occasionally, some amorphous
golden yellow to brown AMG positive signals are noted in the lumen
and basement membrane of some proximal renal tubules. The assay for
estimating the Ag concentration is named the Cetacean Histological
Ag Assay (CHAA), which is a regression model established by the data from
image quantitative analysis of the AMG method and ICP-MS. The
use of AMG with CHAA to localize and semi-quantify heavy metals provides a
convenient methodology for spatio-temporal and cross-species

*You can get more information in https://www.jove.com/video/58232/
<https://www.jove.com/video/58232/> or by email: heerolee1104 at gmail.com
<heerolee1104 at gmail.com>*

This method has been used in another article previously published in
environmental pollution: Investigation of silver (Ag) deposition in tissues
from stranded cetaceans by autometallography (AMG)



Wen-Ta Li, DVM, PhD
Veterinary Pathologist, Graduate Institute of Molecular and Comparative
Pathobiology, National Taiwan University
Veterinarian/Director, Taiwan Cetacean Society
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