[MARMAM] Fw: New Paper on a rapid detection tool for identifying red fox DNA in harbour porpoise carcasses
klassen at uvic.ca
Fri Sep 8 15:16:11 PDT 2017
Re-submission, due to problem with the mailing-list.
From: van Neer, Abbo <Abbo.van.Neer at tiho-hannover.de>
Sent: September 4, 2017 3:04 AM
To: marmam at lists.uvic.ca
Subject: New Paper on a rapid detection tool for identifying red fox DNA in harbour porpoise carcasses
on behalf of all authors I'm pleased to announce the publication of the following article:
Loop-mediated isothermal amplification (LAMP) assay-A rapid detection tool for identifying red fox (Vulpes vulpes) DNA in the carcasses of harbour porpoises (Phocoena phocoena)
Heers, T., van Neer, A., Becker, A., Grilo, M. L., Siebert, U., & Abdulmawjood, A. (2017). PLOS ONE, 12(9), e0184349. doi:10.1371/journal.pone.0184349
Carcasses of wild animals are often visited by different scavengers. However, determining which scavenger caused certain types of bite marks is particularly difficult and knowledge thereof is lacking. Therefore, a loop-mediated isothermal amplification (LAMP) assay (target sequence cytochrome b) was developed to detect red fox DNA in carcasses of harbour porpoises. The MSwab(tm) method for direct testing without prior DNA isolation was validated. As a detection device, the portable real-time fluorometer Genie® II was used, which yields rapid results and can be used in field studies without huge laboratory equipment. In addition to in vitro evaluation and validation, a stranded and scavenged harbour porpoise carcass was successfully examined for red fox DNA residues. The developed LAMP method is a valuable diagnostic tool for confirming presumable red fox bite wounds in harbour porpoises without further DNA isolation steps.
Please follow the link below to obtain a copy.
Abbo van Neer
Abbo van Neer
University of Veterinary Medicine Hannover, Foundation
Institute for Terrestrial and Aquatic Wildlife Research (ITAW)
? +49 5 11 - 8 56 - 81 62
Fax: +49 5 11 - 8 56 - 81 81
abbo.van.neer at tiho-hannover.de<mailto:abbo.van.neer at tiho-hannover.de>
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