[MARMAM] New Article: Assessing progression of cardiomyopathy in pygmy sperm whales with selenium protein profiling

Colleen Bryan - NOAA Affiliate colleen.bryan at noaa.gov
Wed Jun 14 10:07:31 PDT 2017


We are pleased to announce the new publication:

Bryan C.E., Davis W.C., Ballihaut G., Kilpatrick L.E., McFee W.E., Long
S.E., Bossart G.D., Christopher S.J. 2017.  Selenium protein identification
and profiling by mass spectrometry: A tool to assess progression of
cardiomyopathy in a whale model.  Journal of Trace Elements in Medicine and
Biology.  44:  40-49.  http://dx.doi.org/10.1016/j.jtemb.2017.05.005

Abstract:
Non-ischemic cardiomyopathy is a leading cause of congestive heart failure
and sudden cardiac death in humans and in some cases the etiology of
cardiomyopathy can include the downstream effects of an essential element
deficiency.  Of all mammal species, pygmy sperm whales (*Kogia breviceps*)
present the greatest known prevalence of cardiomyopathy with more than half
of examined individuals indicating the presence of cardiomyopathy from
gross and histo-pathology.  Several factors such as genetics, infectious
agents, contaminants, biotoxins, and inappropriate dietary intake
(vitamins, selenium, mercury, and pro-oxidants), may contribute to the
development of idiopathic cardiomyopathy in K. breviceps.  Due to the
important role Se can play in antioxidant biochemistry and protein
formation, Se protein presence and relative abundance were explored in
cardiomyopathy related cases.  Selenium proteins were separated and
detected by multi-dimension liquid chromatography inductively coupled
plasma mass spectrometry (LC-ICP-MS), Se protein identification was
performed by liquid chromatography electrospray tandem mass spectrometry
(LC-ESI-MS/MS), and Se protein profiles were examined in liver (*n* = 30)
and heart tissue (*n* = 5) by SEC/UV/ICP-MS detection.  Data collected on
selenium proteins was evaluated in the context of individual animal trace
element concentration, life history, and histological information. Selenium
containing protein peak profiles varied in presence and intensity between
animals with no pathological findings of cardiomyopathy and animals
exhibiting evidence of cardiomyopathy.  In particular, one class of
proteins, metallothioneins, was found to be associated with Se and was in
greater abundance in animals with cardiomyopathy than those with no
pathological findings.  Profiling Se species with SEC/ICP-MS proved to be a
useful tool to identify Se protein pattern differences between heart
disease stages in *K. breviceps* and an approach similar to this may be
applied to other species to study Se protein associations with
cardiomyopathy.

The article can be downloaded from:
http://www.sciencedirect.com/science/article/pii/S0946672X16302577
or you can email me for a copy.

Cheers,
Colleen Bryan
-- 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Colleen E. Bryan, Ph.D.

National Institute of Standards and Technology

Hollings Marine Laboratory

331 Fort Johnson Road

Charleston, SC 29412

e-mail:  colleen.bryan at nist.gov

phone:  843-762-8832 <(843)%20762-8832>

fax:  843-762-8742 <(843)%20762-8742>
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