[MARMAM] publication on new dolphin morbillivirus molecular technique
sandro.mazzariol at unipd.it
Tue Jun 14 21:57:10 PDT 2016
Dear colleagues, My co-authors and I are pleased to announce the
*Molecular analysis of dolphin morbillivirus: A new sensitive detection
method****based on nested RT-PCR.* Centelleghe C, Beffagna G, Zanetti R,
Zappulli V, Di Guardo G,Mazzariol S. Abstract: Cetacean Morbillivirus
(CeMV) has been identified as the most pathogenic virusfor cetaceans.
Over the past three decades, this RNA virus has caused several outbreaks
of lethal disease in odontocetes and mysticetes worldwide. Isolationand
identification of CeMV RNA is very challenging in whales because of the
poor preservation status frequently shown by tissues from stranded
animals. Nestedreverse transcription polymerase chain reaction (nested
RT-PCR) is used instead of conventional RT-PCR when it is necessary to
increase the sensitivity and thespecificity of the reaction. This study
describes a new nested RT-PCR technique useful to amplify small amounts
of the cDNA copy of Cetacean morbillivirus (CeMV)when it is present in
scant quantity in whales' biological specimens. This technique was used
to analyze different tissues (lung, brain, spleen and otherlymphoid
tissues) from one under human care seal and seven cetaceans stranded
along the Italian coastline between October 2011 and September 2015.
Awell-characterized, 200 base pair (bp) fragment of the dolphin
Morbillivirus (DMV) haemagglutinin (H) gene, obtained by nested RT-PCR,
was sequenced and used to confirm DMV positivity in all the eight marine
mammals under study. In conclusion, this nested RT-PCR protocol can
represent a sensitive detectionmethod to identify CeMV-positive, poorly
preserved tissue samples. Furthermore, this is also a rather inexpensive
molecular technique, relatively easy to apply.
The paper is available online at the following page:
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