[MARMAM] New publication about humpback whale cell line

Burkard, Michael Michael.Burkard at eawag.ch
Fri Sep 18 04:31:39 PDT 2015


Dear colleagues,

My co-authors and I are pleased to announce the publication of a new paper:

„Establishment of the first humpback whale fibroblast cell lines and their application in chemical risk assessment"

Abstract:
This paper reports the first successful derivation and characterization of humpback whale fibroblast cell lines. Primary fibroblasts were isolated from the dermal connective tissue of skin biopsies, cultured at 37◦C and 5% CO2 in the standard mammalian medium DMEM/F12 supplemented with 10% fetal bovine serum (FBS). Of nine initial biopsies, two cell lines were established from two different animals and designated HuWa1 and HuWa2. The cells have a stable karyotype with 2n = 44, which has commonly been observed in other baleen whale species. Cells were verified as being fibroblasts based on their spindle-shaped morphology, adherence to plastic and positive immunoreaction to vimentin. Population doubling time was determined to be ∼41 h and cells were successfully cryopreserved and thawed. To date, HuWa1 cells have been propagated 30 times. Cells proliferate at the tested temper- atures, 30, 33.5 and 37 ◦ C, but show the highest rate of proliferation at 37◦ C. Short-term exposure to para,para′ -dichlorodiphenyldichloroethylene (p,p′ -DDE), a priority compound accumulating in southern hemisphere humpback whales, resulted in a concentration-dependent loss of cell viability. The effective concentration which caused a 50% reduction in HuWa1 cell viability (EC50 value) was approximately six times greater than the EC50 value for the same chemical measured with human dermal fibroblasts. HuWa1 exposed to a natural, p,p′-DDE-containing, chemical mixture extracted from whale blubber showed dis- tinctively higher sensitivity than to p,p′-DDE alone. Thus, we provide the first cytotoxicological data for humpback whales and with establishment of the HuWa cell lines, a unique in vitro model for the study of the whales’ sensitivity and cellular response to chemicals and other environmental stressors.


A PDF version of the paper is available online at:
http://authors.elsevier.com/a/1Rgvp_,OE92iql

or

https://www.researchgate.net/publication/281641631_Establishment_of_the_first_humpback_whale_fibroblast_cell_lines_and_their_application_in_chemical_risk_assessmen

or via e-mail request to michael.burkard at eawag.ch<mailto:michael.burkard at eawag.ch>

Cheers,
Michael Burkard

[cid:61A2A85B-F56E-4AB5-85C9-260FAD2F1118 at guests.emp-eaw.ch]

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.uvic.ca/pipermail/marmam/attachments/20150918/75723f58/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: PastedGraphic-1.tiff
Type: image/tiff
Size: 104802 bytes
Desc: PastedGraphic-1.tiff
URL: <http://lists.uvic.ca/pipermail/marmam/attachments/20150918/75723f58/attachment-0001.tiff>


More information about the MARMAM mailing list