[MARMAM] New publication: A protein A/G indirect ELISA for the detection of anti-Brucella antibodies in Arctic wildlife
Nymo Ingebjørg Helena
Ingebjorg.Nymo at nvh.no
Tue Apr 16 12:45:05 PDT 2013
We have recently published a new publication in Journal of Veterinary Diagnostic Investigation: A protein A/G indirect enzyme-linked immunosorbent assay for the detection of anti-Brucella antibodies in Arctic wildlife.
Avaliable online at: http://vdi.sagepub.com/content/early/2013/03/28/1040638713485073.abstract
The paper validates an serological method for anti-Brucella antibodies in Arctic wildlife, spesifically in hooded seal, various whales, polar bear and reindeer. We also present seroprevalences for the different species.
A species-independent indirect enzyme-linked immunosorbent assay (iELISA) based on chimeric protein A/G was established for the detection of anti-Brucella antibodies in Arctic wildlife species and compared to previously established brucellosis serological tests for hooded seals (Cystophora cristata), minke whales (Balaenoptera acutorostrata), sei whales (Balaenoptera borealis), fin whales (Balaenoptera physalus), and polar bears (Ursus maritimus), as well as bacteriology results for reindeer and caribou (Rangifer tarandus sp.). The protein A/G iELISA results were consistent with the other serological tests with Cohen kappa values between 0.47 and 0.92, and the protein A/G iELISA can thus offer a technically simple method for these species yielding results consistent with established brucellosis serological tests. Receiver operator characteristics analysis proved that the reindeer and caribou protein A/G iELISA results were consistent with the bacteriological gold standard with an area under the curve of 0.99, and the protein A/G iELISA was thus validated as a sensitive and specific serological method for the detection of anti-Brucella antibodies in reindeer and caribou. The binding of the antibodies from the respective species to protein A and G were also evaluated in the iELISA. The antibodies from hooded seals and polar bears reacted stronger to protein A than to G. The sei whale, fin whale, reindeer, and caribou antibodies reacted stronger to protein G than to A. The minke whale antibodies reacted to both protein A and G. There was a strong correlation (rs = 0.88–0.98) between the optical density results obtained with the iELISA with protein A/G and protein A or G, showing that protein A/G is as well suited as protein A or G for the detection of anti-Brucella antibodies in these species with the iELISA.
Best wishes from the north,
Ingebjørg H. Nymo (ingebjorg.nymo at nvh.no<mailto:ingebjorg.nymo at nvh.no>)
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