[MARMAM] MRI comparison of live and postmortem sea lions with domoic acid toxicosis
emontie at MARINE.USF.EDU
Thu Sep 23 17:24:20 PDT 2010
I would like to bring to your attention the following paper, "Magnetic
resonance imaging quality and volumes of brain structures from live and
postmortem imaging of California sea lions with clinical signs of domoic
acid toxicosis". This manuscript was published in Diseases of Aquatic
Organisms. It is available as an open access document on the following
web page: http://www.int-res.com/abstracts/dao/v91/n3/.
ABSTRACT: Our goal in this study was to compare magnetic resonance
images and volumes of brain structures obtained alive versus postmortem
of California sea lions Zalophus californianus exhibiting clinical signs
of domoic acid (DA) toxicosis and those exhibiting normal behavior.
Proton density- (PD) and T2-weighted images of postmortem-intact brains,
up to 48 h after death, provided similar quality to images acquired from
live sea lions. Volumes of gray matter (GM) and white matter (WM) of the
cerebral hemispheres were similar to volumes calculated from images
acquired when the sea lions were alive. However, cerebrospinal fluid
(CSF) volumes decreased due to leakage. Hippocampal volumes from
postmortem-intact images were useful for diagnosing unilateral and
bilateral atrophy, consequences of DA toxicosis. These volumes were
similar to the volumes in the live sea lion studies, up to 48 h
postmortem. Imaging formalin-fixed brains provided some information on
brain structure; however, images of the hippocampus and surrounding
structures were of poorer quality compared to the images acquired alive
and postmortem-intact. Despite these issues, volumes of cerebral GM and
WM, as well as the hippocampus, were similar to volumes calculated from
images of live sea lions and sufficient to diagnose hippocampal atrophy.
Thus, postmortem MRI scanning (either intact or formalin-fixed) with
volumetric analysis can be used to investigate the acute, chronic and
possible developmental effects of DA on the brain of California sea lions.
If you have any questions about the paper or have trouble accessing this
website, please feel free to contact me.
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