[MARMAM] MRI comparison of live and postmortem sea lions with domoic acid toxicosis

Eric Montie emontie at MARINE.USF.EDU
Thu Sep 23 17:24:20 PDT 2010

Dear Colleagues,
I would like to bring to your attention the following paper, "Magnetic 
resonance imaging quality and volumes of brain structures from live and 
postmortem imaging of California sea lions with clinical signs of domoic 
acid toxicosis". This manuscript was published in Diseases of Aquatic 
Organisms.  It is available as an open access document on the following 
web page: http://www.int-res.com/abstracts/dao/v91/n3/.

ABSTRACT: Our goal in this study was to compare magnetic resonance 
images and volumes of brain structures obtained alive versus postmortem 
of California sea lions Zalophus californianus exhibiting clinical signs 
of domoic acid (DA) toxicosis and those exhibiting normal behavior. 
Proton density- (PD) and T2-weighted images of postmortem-intact brains, 
up to 48 h after death, provided similar quality to images acquired from 
live sea lions. Volumes of gray matter (GM) and white matter (WM) of the 
cerebral hemispheres were similar to volumes calculated from images 
acquired when the sea lions were alive. However, cerebrospinal fluid 
(CSF) volumes decreased due to leakage. Hippocampal volumes from 
postmortem-intact images were useful for diagnosing unilateral and 
bilateral atrophy, consequences of DA toxicosis. These volumes were 
similar to the volumes in the live sea lion studies, up to 48 h 
postmortem. Imaging formalin-fixed brains provided some information on 
brain structure; however, images of the hippocampus and surrounding 
structures were of poorer quality compared to the images acquired alive 
and postmortem-intact. Despite these issues, volumes of cerebral GM and 
WM, as well as the hippocampus, were similar to volumes calculated from 
images of live sea lions and sufficient to diagnose hippocampal atrophy. 
Thus, postmortem MRI scanning (either intact or formalin-fixed) with 
volumetric analysis can be used to investigate the acute, chronic and 
possible developmental effects of DA on the brain of California sea lions.

If you have any questions about the paper or have trouble accessing this 
website, please feel free to contact me.

Best regards,
Eric Montie

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