[MARMAM] New paper: Serological Diagnosis of Brucella Infections in Odontocetes (Gabriela Hernández- Mora)

Gaby Hernandez gabbytica at gmail.com
Mon Jun 15 21:11:10 PDT 2009


Dear MARMAM readers,



I am pleased to announce the publication of the following article in
Clinical and Vaccine Immunology, (abstract below):





Hernández-Mora G, Manire C. A, González-Barrientos R., Barquero-Calvo E.,
Guzmán-Verri C., Staggs L., Thompson R., Chaves-Olarte E.,* and E.
Moreno. *Serological
Diagnosis of *Brucella *Infections in Odontocetes. 2009. Clin. Vaccine
Immunol. Jun  906–915







*Abstract:*

* *

*Brucella ceti *causes disease in Odontoceti. The absence of control serum
collections and the diversity of cetaceans have hampered the standardization
of serological tests for the diagnosis of cetacean brucellosis. Without a
“gold” standard for sensitivity and specificity determination, an
alternative approach was followed.  We designed an indirect enzyme-linked
immunosorbent assay (iELISA) that recognizes immunoglobulins G (IgGs) from
17 odontocete species as a single group. For the standardization, we
used *Brucella
melitensis *and *Brucella abortus *lipopolysaccharides, serum samples from
seven resident odontocetes with no history of infectious disease displaying
negative rose bengal test (RBT) reactions, and serum samples from seven
dolphins infected with *B. ceti. *We compared the performance of the iELISA
with those of the protein G ELISA (gELISA), the competitive ELISA (cELISA),
and the immunofluorescence (IF) and dot blot (DB) tests, using 179
odontocete serum samples and RBT as the reference. The diagnostic potential
based on sensitivity and specificity of the iELISA was superior to that of
gELISA and cELISA. The correlation and agreement between the iELISA and the
gELISA were relatively good (*R*i/g 2 = 0.65 and ĸ i/g = 0.66,
respectively), while the correlation and agreement of these two ELISAs with
cELISA were low (*R*i/c2 = 0.46, *R*g/c2 = 0.37 and ĸ i/c = 0.62, ĸ
g/c = 0.42).
In spite of using the same anti-odontocete IgG antibody, the iELISA was more
specific than were the IF and DB tests. An association between high antibody
titers and the presence of neurological symptoms in dolphins was observed.
The prediction is that iELISA based on broadly cross-reacting anti-dolphin
IgG antibody would be a reliable test for the diagnosis of brucellosis in
odontocetes, including families not covered in this study.



Pdf copies are available on request: ghernandez at fundacionketo.org



Best regards,



Gabriela Hernández Mora

Investigation Program in Tropical Diseases (PIET)

Universidad Nacional,

P.O.Box 304-3000, Heredia

Costa Rica

www.fundacionketo.org
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